Journal: Journal of Otolaryngology - Head & Neck Surgery

Article Title: Prognostic significance of cell cycle-associated proteins p16, pRB, cyclin D1 and p53 in resected oropharyngeal carcinoma

doi: 10.1186/s40463-018-0298-3

Figure Lengend Snippet: Expression of cell cycle-associated proteins in oropharyngeal squamous cell carcinoma. Histological findings ( a , b ) and expression of the p16 INK4a ( c , d ), pRB ( e , f ), cyclin D1 ( g , h ) and p53 ( i , j ) proteins in representative HR-HPV positive ( b , d , f , h , j ) and HR-HPV negative OPSCC ( a , c , e , g , i ). Original magnification, 400×

Article Snippet: Stainings were performed on a Leica BOND MAX instrument (Leica) using the Bond Polymer Refine detection kit (Leica) and the following primary antibodies: p16 INK4a (clone E6H4, dilution 1:10, 30 min; MTM Laboratories, Heidelberg, Germany), pRB (clone 13A10, dilution 1:100, 15 min; Novocastra), cyclin D1 (clone SP4, dilution 1:50, 30 min; ThermoScientific), and p53 (clone DO-7, dilution 1:150, 30 min; Dako).

Techniques: Expressing

Journal: Journal of Otolaryngology - Head & Neck Surgery

Article Title: Prognostic significance of cell cycle-associated proteins p16, pRB, cyclin D1 and p53 in resected oropharyngeal carcinoma

doi: 10.1186/s40463-018-0298-3

Figure Lengend Snippet: Association between cell cycle protein expression and HR-HPV DNA status

Article Snippet: Stainings were performed on a Leica BOND MAX instrument (Leica) using the Bond Polymer Refine detection kit (Leica) and the following primary antibodies: p16 INK4a (clone E6H4, dilution 1:10, 30 min; MTM Laboratories, Heidelberg, Germany), pRB (clone 13A10, dilution 1:100, 15 min; Novocastra), cyclin D1 (clone SP4, dilution 1:50, 30 min; ThermoScientific), and p53 (clone DO-7, dilution 1:150, 30 min; Dako).

Techniques: Expressing

Journal: Journal of Otolaryngology - Head & Neck Surgery

Article Title: Prognostic significance of cell cycle-associated proteins p16, pRB, cyclin D1 and p53 in resected oropharyngeal carcinoma

doi: 10.1186/s40463-018-0298-3

Figure Lengend Snippet: Overall survival after resection in patients with oropharyngeal squamous cell carcinoma. Kaplan-Meier analyses revealed associations between improved survival and HR-HPV DNA positivity ( a ), high p16 INK4a ( b ), low pRB ( c ), high p16 INK4a /low pRB signature ( d ), low cyclin D1 ( e ), and low p53 ( f )

Article Snippet: Stainings were performed on a Leica BOND MAX instrument (Leica) using the Bond Polymer Refine detection kit (Leica) and the following primary antibodies: p16 INK4a (clone E6H4, dilution 1:10, 30 min; MTM Laboratories, Heidelberg, Germany), pRB (clone 13A10, dilution 1:100, 15 min; Novocastra), cyclin D1 (clone SP4, dilution 1:50, 30 min; ThermoScientific), and p53 (clone DO-7, dilution 1:150, 30 min; Dako).

Techniques:

Journal: Journal of Otolaryngology - Head & Neck Surgery

Article Title: Prognostic significance of cell cycle-associated proteins p16, pRB, cyclin D1 and p53 in resected oropharyngeal carcinoma

doi: 10.1186/s40463-018-0298-3

Figure Lengend Snippet: Univariate analysis of overall and disease-specific survival for patients with OPSCC patients treated with resection

Article Snippet: Stainings were performed on a Leica BOND MAX instrument (Leica) using the Bond Polymer Refine detection kit (Leica) and the following primary antibodies: p16 INK4a (clone E6H4, dilution 1:10, 30 min; MTM Laboratories, Heidelberg, Germany), pRB (clone 13A10, dilution 1:100, 15 min; Novocastra), cyclin D1 (clone SP4, dilution 1:50, 30 min; ThermoScientific), and p53 (clone DO-7, dilution 1:150, 30 min; Dako).

Techniques:

Journal: Journal of Otolaryngology - Head & Neck Surgery

Article Title: Prognostic significance of cell cycle-associated proteins p16, pRB, cyclin D1 and p53 in resected oropharyngeal carcinoma

doi: 10.1186/s40463-018-0298-3

Figure Lengend Snippet: Multivariate analysis of overall and disease-specific survival for patients with OPSCC patients treated with resection

Article Snippet: Stainings were performed on a Leica BOND MAX instrument (Leica) using the Bond Polymer Refine detection kit (Leica) and the following primary antibodies: p16 INK4a (clone E6H4, dilution 1:10, 30 min; MTM Laboratories, Heidelberg, Germany), pRB (clone 13A10, dilution 1:100, 15 min; Novocastra), cyclin D1 (clone SP4, dilution 1:50, 30 min; ThermoScientific), and p53 (clone DO-7, dilution 1:150, 30 min; Dako).

Techniques:

Journal: Chinese Journal of Cancer Research

Article Title: Aberration of the Wnt signaling pathway in pulmonary fatal adenocarcinoma: a case report

doi: 10.3978/j.issn.1000-9604.2014.02.05

Figure Lengend Snippet: Immunohistochemical staining of fatal adenocarcinoma. (A) Elevated nuclear/cytoplasmic expression of β-catenin in solid nests (black arrow, ×100); (B) Elevated nuclear/cytoplasmic expression of β-catenin in solid nests (black arrow, ×400); Positive expressions of Wnt1 (C), c-Myc (D), Cyclin D1 (E), and MMP7 (F) were especially prominent in solid nests, but not in glandular structures (×100).

Article Snippet: The sections were incubated with the rabbit polyclonal antibody for Wnt1 (H-89, Santa Cruz, dilution 1 : 100), the mouse monoclonal antibody for β-catenin (5H10, Zymed Laboratorys, dilution 1 : 1,000), the mouse monoclonal antibody for c-Myc (9E11, Santa Cruz, dilution 1 : 5,000), the rabbit monoclonal antibody for Cyclin D1 (SP4, Thermo Fisher Scientific, dilution 1 : 100) and the mouse monoclonal antibody for MMP7 (JL07, Santa Cruz, dilution 1 : 100).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Journal of Carcinogenesis

Article Title: Expression of epidermal growth factor receptor, p53, Bcl2, vascular endothelial growth factor, cyclooxygenase-2, cyclin D1, human epidermal receptor-2 and Ki-67: Association with clinicopathological profiles and outcomes in gallbladder carcinoma

doi: 10.4103/1477-3163.139450

Figure Lengend Snippet: Representative strong positive immunohistochemical staining, labeled with Diaminobenzidine, for (a) epidermal growth factor receptor (×100), (b) p53 (×40) (c) Bcl2 (×100), (d) vascular endothelial growth factor (×100), (e) cyclooxygenase-2 (×100), (f) cyclin D1 (×40), (g) human epidermal receptor-2 (×400), (h) Ki-67 (×100)

Article Snippet: Rabbit anti-human monoclonal antibody to EGFR (Clone SP4, Diagnostics Biosystems, USA), cyclin D1 (SP4, Biocare Medical, USA), cox-2 (Clone SP21, Biocare Medical, USA), and mouse anti-human monoclonal antibodies to human VEGF (Clone VG1, DakoCytomation, Denmark), Bcl2 (Clone 124, Dako, Denmark), p53 (DO-7, Novacastra Laboratories), Ki-67 antigen (Clone MIB-1, Dako, Denmark) were used.

Techniques: Immunohistochemical staining, Staining, Labeling

Journal: Journal of Carcinogenesis

Article Title: Expression of epidermal growth factor receptor, p53, Bcl2, vascular endothelial growth factor, cyclooxygenase-2, cyclin D1, human epidermal receptor-2 and Ki-67: Association with clinicopathological profiles and outcomes in gallbladder carcinoma

doi: 10.4103/1477-3163.139450

Figure Lengend Snippet: Kaplan–Meier curves for overall survival according to the biomarker overexpression (epidermal growth factor receptor, p53, Bcl2, vascular endothelial growth factor, cyclooxygenase-2, cyclin D1, human epidermal receptor-2) and clinicpathological factors (stage and tumor differentiation)

Article Snippet: Rabbit anti-human monoclonal antibody to EGFR (Clone SP4, Diagnostics Biosystems, USA), cyclin D1 (SP4, Biocare Medical, USA), cox-2 (Clone SP21, Biocare Medical, USA), and mouse anti-human monoclonal antibodies to human VEGF (Clone VG1, DakoCytomation, Denmark), Bcl2 (Clone 124, Dako, Denmark), p53 (DO-7, Novacastra Laboratories), Ki-67 antigen (Clone MIB-1, Dako, Denmark) were used.

Techniques: Biomarker Assay, Over Expression

Journal: Molecular cell

Article Title: Transient Hysteresis in CDK4/6 Activity Underlies Passage of the Restriction Point in G1

doi: 10.1016/j.molcel.2019.08.020

Figure Lengend Snippet: (A) Schematic highlighting possible threshold or cell-cycle timing-dependent maintenance of Rb hyperphosphorylation by cyclin E/A-CDK following acute CDK4/6 inhibition.

Article Snippet: α-cyclin D1 rabbit mAb [SP4] , Thermo Scientific , Cat#RM-9104-S0, RRID:AB_149914.

Techniques: Inhibition

Journal: Molecular cell

Article Title: Transient Hysteresis in CDK4/6 Activity Underlies Passage of the Restriction Point in G1

doi: 10.1016/j.molcel.2019.08.020

Figure Lengend Snippet: (A) Cycling MCF-10A expressing cyclin E/A-CDK activity reporter were live-cell-imaged and treated with CDK4/6i or vehicle for 15 min, followed by fixing andstaining for normalized Rb(p-S807/S811). Scale bar, 5 μm. Single-cell correlation of cyclin E/A-CDK activity just prior to drug treatment versus the resulting phospho-Rb signal. n = 7,000 cells per condition.

Article Snippet: α-cyclin D1 rabbit mAb [SP4] , Thermo Scientific , Cat#RM-9104-S0, RRID:AB_149914.

Techniques: Expressing, Activity Assay

Journal: Molecular cell

Article Title: Transient Hysteresis in CDK4/6 Activity Underlies Passage of the Restriction Point in G1

doi: 10.1016/j.molcel.2019.08.020

Figure Lengend Snippet: (A) MCF-10A expressing cyclin E/A-CDK activity reporter and APC/C degron reporter. Left: 50 sample traces with automated detection of E/A activation and APC/C inactivation illustrated. Middle: 50 sample traces when mitogen removed 6 h after release. High future E/A activity (green traces) defined as >1.0 at 24 h post-release. High future APC/C deg (green traces) defined as increasing >10 a.u. from drug treatment to 24 h post-release. Error is SD of n = 3. Right: percentage of cells with E/A-on (green) or APC/C-off (orange) since mitogen release. In parallel, mitogens removed at various times following mitogen release, and the percentage of cells that will eventually inactivate APC/C by 24 h post-mitogen release is shown (black). Error bars are SD; n > 580 cells per condition; n = 3 replicates.

Article Snippet: α-cyclin D1 rabbit mAb [SP4] , Thermo Scientific , Cat#RM-9104-S0, RRID:AB_149914.

Techniques: Expressing, Activity Assay, Activation Assay

Journal: Molecular cell

Article Title: Transient Hysteresis in CDK4/6 Activity Underlies Passage of the Restriction Point in G1

doi: 10.1016/j.molcel.2019.08.020

Figure Lengend Snippet: (A) MCF-10A treated as illustrated and stained and quantified for nuclear cyclin D1 expression. Scale bar, 25 μm. Representative of n = 3 replicates.

Article Snippet: α-cyclin D1 rabbit mAb [SP4] , Thermo Scientific , Cat#RM-9104-S0, RRID:AB_149914.

Techniques: Staining, Expressing

Journal: Molecular cell

Article Title: Transient Hysteresis in CDK4/6 Activity Underlies Passage of the Restriction Point in G1

doi: 10.1016/j.molcel.2019.08.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: α-cyclin D1 rabbit mAb [SP4] , Thermo Scientific , Cat#RM-9104-S0, RRID:AB_149914.

Techniques: Recombinant, Protease Inhibitor, Negative Control, Software

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Association of CCND1 G870A polymorphism (rs9344) and the levels of CCND1 mRNA expression in thyroid tumors. ( a ) The AA genotype is associated with high levels of CCND1b mRNA but not with those of CCND1a mRNA. ( b ) The distribution of rs9344 genotypes with respect to the various types of thyroid tumors. NH, nodular hyperplasia; FA, follicular adenoma; NIFTP, noninvasive follicular thyroid neoplasm with papillary-like nuclear features; PTC, papillary thyroid carcinoma; FTC, follicular thyroid carcinoma; PDTC, poorly differentiated thyroid carcinoma; MTC, medullary thyroid carcinoma.

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Expressing

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Expression of CCND1 mRNA isoforms relative to that of GAPDH in various types of thyroid tumors. NH, nodular hyperplasia; FA, follicular adenoma; NIFTP, noninvasive follicular thyroid neoplasm with papillary-like nuclear features; PTC, papillary thyroid carcinoma; FTC, follicular thyroid carcinoma; PDTC, poorly differentiated thyroid carcinoma; MTC, medullary thyroid carcinoma.

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Expressing

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Immunohistochemical staining of cyclin D1a and cyclin D1b in various types of thyroid tumors (×400).

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Expression of cyclin D1a and cyclin D1b in thyroid tumors from cohort 1 and cohort 2.

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Expressing, Variant Assay

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Heterogeneous immunostaining for cyclin D1b in papillary thyroid carcinoma. ( a ) Low magnification (×12) shows a classical papillary thyroid carcinoma with insets corresponding to high-power view of immunostaining for cyclin D1b shown in ( b – d ). ( b ) The central areas show a patchy staining pattern (×400). ( c – d ) Tumor cells at the invasive front show a diffuse staining pattern (×400).

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Immunostaining, Staining

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Correlation between clinicopathologic features and expression of CCND1 mRNA isoforms and cyclin D1b protein in 170 patients with papillary thyroid carcinoma. AJCC: American Joint Committee on Cancer.

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Expressing, Variant Assay, Mutagenesis

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Correlation between clinicopathologic features and expression of mRNA CCND1 isoforms and cyclin D1b protein in 125 patients with classic variant of papillary thyroid carcinoma.

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Expressing, Variant Assay, Mutagenesis

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Expression of CCND1b mRNA and cyclin D1b protein in an independent cohort of noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) and invasive encapsulated follicular variant of papillary thyroid carcinoma.

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Expressing, Variant Assay, Mutagenesis

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Immunohistochemistry for cyclin D1a and cyclin D1b in noninvasive follicular thyroid neoplasm with papillary-like nuclear features ( a – d ) and invasive encapsulated follicular variant of papillary thyroid carcinoma ( e – h ). ( a ) Image of noninvasive follicular thyroid neoplasm with papillary-like nuclear features shows clear demarcation and follicular growth pattern (×40). ( b ) This tumor shows the nuclear features of papillary thyroid carcinoma characterized by nuclear enlargement, overlapping, and membrane irregularities (×400). ( c ) Immunohistochemical staining positive for cyclin D1a ( c , ×100) and negative for cyclin D1b ( d , ×100). ( e ) Histologic features of an invasive encapsulated follicular variant of papillary thyroid carcinoma (×40). ( f ) Positive immunostaining for cyclin D1a demarcates the site of invasive growth (indicated by arrows, ×100). High magnification (×400) showing positive immunostaining for cyclin D1a ( g ) and cyclin D1b ( h ).

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Immunohistochemistry, Variant Assay, Membrane, Immunohistochemical staining, Staining, Immunostaining

Journal: Cancers

Article Title: CCND1 Splice Variant as A Novel Diagnostic and Predictive Biomarker for Thyroid Cancer

doi: 10.3390/cancers10110437

Figure Lengend Snippet: Noninvasive encapsulated classic papillary thyroid carcinoma with predominant follicular growth and BRAF V600E mutation. ( a ) Low magnification shows a well-circumscribed follicular tumor measuring 1.1 cm in greatest dimension (×12). ( b ) The tumor is clearly demarcated from normal thyroid tissue by a fibrous capsule (×100). ( c ) The nuclear features of tumor cells show nuclear enlargement, membrane irregularities, pseudoinclusions (indicated by arrows), and grooves (×400). ( d ) Follicles with abortive papillae are observed (×400). Immunohistochemistry shows diffuse positivity for BRAF VE1 ( e ) and focal positivity for cyclin D1b ( f ).

Article Snippet: The rabbit monoclonal cyclin D1 (clone SP4, Cat. No. 241R-18) antibody for in vitro diagnostic use was purchased from Cell Marque (Rocklin, CA, USA).

Techniques: Mutagenesis, Membrane, Immunohistochemistry